BS ISO 17601:2016 Historical

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Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

standard by British Standard / International Organization for Standardization , 01/31/2016

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BS ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.

It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.


Cross References:
ISO 10381-6
ISO 11063


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Document History

  1. BS EN ISO 17601:2025


    Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative polymerase chain reaction (qPCR) from DNA directly extracted from soil

    • Most Recent

  2. BS EN ISO 17601:2018


    Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

    • Historical Version

  3. BS ISO 17601:2016

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    Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

    • Historical Version

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Product Details

Published:

01/31/2016

ISBN(s):

9780580767180

Number of Pages:

40

File Size:

1 file

Same As:

ISO 17601:2016

Product Code(s):

30253329, 30253329, 30253329

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